@article {Radzinski2018,
	title = {Temporal profiling of redox-dependent heterogeneity in single cells},
	journal = {eLife},
	volume = {7},
	year = {2018},
	note = {cited By 21},
	abstract = {Cellular redox status affects diverse cellular functions, including proliferation, protein homeostasis, and aging. Thus, individual differences in redox status can give rise to distinct subpopulations even among cells with identical genetic backgrounds. Here, we have created a novel methodology to track redox status at single cell resolution using the redox-sensitive probe Grx1-roGFP2. Our method allows identification and sorting of sub-populations with different oxidation levels in either the cytosol, mitochondria or peroxisomes. Using this approach, we defined a redox-dependent heterogeneity of yeast cells and characterized growth, as well as proteomic and transcriptomic profiles of distinctive redox subpopulations. We report that, starting in late logarithmic growth, cells of the same age have a bi-modal distribution of oxidation status. A comparative proteomic analysis between these populations identified three key proteins, Hsp30, Dhh1, and Pnc1, which affect basal oxidation levels and may serve as first line of defense proteins in redox homeostasis. {\textcopyright} Radzinski et al.},
	doi = {10.7554/eLife.37623},
	url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85051931605\&doi=10.7554\%2feLife.37623\&partnerID=40\&md5=64905fb7eaecf482661ac978d8a9762c},
	author = {Radzinski, M. and Fassler, R. and Yogev, O. and Breuer, W. and Shai, N. and Gutin, J. and Ilyas, S. and Geffen, Y. and Tsytkin-Kirschenzweig, S. and Nahmias, Y. and Ravid, T. and Friedman, N. and Schuldiner, M. and Reichmann, D.}
}
