@article {Ordov{\'a}s2015918,
	title = {Efficient recombinase-mediated cassette exchange in hPSCs to study the hepatocyte lineage reveals AAVS1 locus-mediated transgene inhibition},
	journal = {Stem Cell Reports},
	volume = {5},
	number = {5},
	year = {2015},
	note = {cited By 103},
	pages = {918-931},
	abstract = {Tools for rapid and efficient transgenesis in "safe harbor" loci in an isogenic context remain important to exploit the possibilities of human pluripotent stem cells (hPSCs). We created hPSC master cell lines suitable for FLPe recombinase-mediated cassette exchange (RMCE) in the AAVS1 locus that allow generation of transgenic lines within 15 days with 100\% efficiency and without random integrations. Using RMCE, we successfully incorporated several transgenes useful for lineage identification, cell toxicity studies, and gene overexpression to study the hepatocyte lineage. However, we observed unexpected and variable transgene expression inhibition in vitro, due to DNA methylation and other unknown mechanisms, both in undifferentiated hESC and differentiating hepatocytes. Therefore, the AAVS1 locus cannot be considered a universally safe harbor locus for reliable transgene expression in vitro, and using it for transgenesis in hPSC will require careful assessment of the function of individual transgenes. {\textcopyright} 2015 The Authors.},
	doi = {10.1016/j.stemcr.2015.09.004},
	url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84946714665\&doi=10.1016\%2fj.stemcr.2015.09.004\&partnerID=40\&md5=a12856fa562f6d898ce16ca1b0bb3c34},
	author = {Ordov{\'a}s, L. and Boon, R. and Pistoni, M. and Chen, Y. and Wolfs, E. and Guo, W. and Sambathkumar, R. and Bobis-Wozowicz, S. and Helsen, N. and Vanhove, J. and Berckmans, P. and Cai, Q. and Vanuytsel, K. and Eggermont, K. and Vanslembrouck, V. and Schmidt, B.Z. and Raitano, S. and Van Den Bosch, L. and Nahmias, Y. and Cathomen, T. and Struys, T. and Verfaillie, C.M.}
}
