@article {Moussaieff2015392,
	title = {Glycolysis-mediated changes in acetyl-CoA and histone acetylation control the early differentiation of embryonic stem cells},
	journal = {Cell Metabolism},
	volume = {21},
	number = {3},
	year = {2015},
	note = {cited By 504},
	pages = {392-402},
	abstract = {Loss of pluripotency is a gradual event whose initiating factors are largely unknown. Here we report the earliest metabolic changes induced during the first hours of differentiation. High-resolution NMR identified 44 metabolites and a distinct metabolic transition occurring during early differentiation. Metabolic and transcriptional analyses showed that pluripotent cells produced acetyl-CoA through glycolysis and rapidly lost this function during differentiation. Importantly, modulation of glycolysis blocked histone deacetylation and differentiation in human and mouse embryonic stem cells. Acetate, a precursor of acetyl-CoA, delayed differentiation and blocked early histone deacetylation in a dose-dependent manner. Inhibitors upstream of acetyl-CoA caused differentiation of pluripotent cells, while those downstream delayed differentiation. Our results show a metabolic switch causing a loss of histone acetylation and pluripotent state during the first hours of differentiation. Our data highlight the important role metabolism plays in pluripotency and suggest that a glycolytic switch controlling histone acetylation can release stem cells from pluripotency. {\textcopyright} 2015 Elsevier Inc.},
	doi = {10.1016/j.cmet.2015.02.002},
	url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84924369505\&doi=10.1016\%2fj.cmet.2015.02.002\&partnerID=40\&md5=a4ea30058a0c4597f967ecb6ad53f490},
	author = {Moussaieff, A. and Rouleau, M. and Kitsberg, D. and Cohen, M. and Levy, G. and Barasch, D. and Nemirovski, A. and Shen-Orr, S. and Laevsky, I. and Amit, M. and Bomze, D. and Elena-Herrmann, B. and Scherf, T. and Nissim-Rafinia, M. and Kempa, S. and Itskovitz-Eldor, J. and Meshorer, E. and Aberdam, D. and Nahmias, Y.}
}
