@article {Cho2008898,
	title = {Homogeneous differentiation of hepatocyte-like cells from embryonic stem cells: Applications for the treatment of liver failure},
	journal = {FASEB Journal},
	volume = {22},
	number = {3},
	year = {2008},
	note = {cited By 65},
	pages = {898-909},
	abstract = {One of the major hurdles of cellular therapies for the treatment of liver failure is the low availability of functional human hepatocytes. While embryonic stem (ES) cells represent a potential cell source for therapy, current methods for differentiation result in mixed cell populations or low yields of the cells of interest. Here we describe a rapid, direct differentiation method that yields a homogeneous population of endoderm-like cells with 95\% purity. Mouse ES cells cultured on top of collagen-sandwiched hepatocytes differentiated and proliferated into a uniform and homogeneous cell population of endoderm-like cells. The endoderm-like cell population was positive for Foxa2, Sox17, and AFP and could be further differentiated into hepatocyte-like cells, demonstrating hepatic morphology, functionality, and gene and protein expression. Incorporating the hepatocyte-like cells into a bioartificial liver device to treat fulminant hepatic failure improved animal survival, thereby underscoring the therapeutic potential of these cells. {\textcopyright} FASEB.},
	doi = {10.1096/fj.06-7764com},
	url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-40449118677\&doi=10.1096\%2ffj.06-7764com\&partnerID=40\&md5=24702a80d10bda505dcd8bde707685a7},
	author = {Cho, C.H. and Parashurama, N. and Park, E.Y.H. and Suganuma, K. and Nahmias, Y. and Park, J. and Tilles, A.W. and Berthiaume, F. and Yarmush, M.L.}
}
